multiplex identifier tag Search Results


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Cytiva Europe resource source identifier histrap hp columns cytiva
Resource Source Identifier Histrap Hp Columns Cytiva, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad resource source identifier antibodies mouse anti v5 bio rad
Figure 1. Intestinal progenitor cells contain P-bodies (A–D) Adult posterior midguts (PMGs) stained for EBs <t>(V5,</t> white), progenitor cells (HRP, green), all cells (DAPI, blue), and either EDC3, GE-1, Me31B, or TRAL (red). (A0)–(D0) are enlargements with EBs labeled (asterisks). (E) PMG stained for TRAL (red), Me31B (green), and DAPI (blue). (F, H, and J) Super-resolution micrographs of progenitor cells stained for EDC3, Me31B, or TRAL pseudocolored using intensity scale in (J); insets are from boxed regions (scale bar, 0.125 mm). (G, I, and K) Pixel-by-pixel intensity profiles along the green dotted line shown in insets. Full genotypes are listed in Data S1F. See also Figure S1.
Resource Source Identifier Antibodies Mouse Anti V5 Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson single-cell multiplexing kit
Figure 1. Intestinal progenitor cells contain P-bodies (A–D) Adult posterior midguts (PMGs) stained for EBs <t>(V5,</t> white), progenitor cells (HRP, green), all cells (DAPI, blue), and either EDC3, GE-1, Me31B, or TRAL (red). (A0)–(D0) are enlargements with EBs labeled (asterisks). (E) PMG stained for TRAL (red), Me31B (green), and DAPI (blue). (F, H, and J) Super-resolution micrographs of progenitor cells stained for EDC3, Me31B, or TRAL pseudocolored using intensity scale in (J); insets are from boxed regions (scale bar, 0.125 mm). (G, I, and K) Pixel-by-pixel intensity profiles along the green dotted line shown in insets. Full genotypes are listed in Data S1F. See also Figure S1.
Single Cell Multiplexing Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio resource source identifier antibodies mouse monoclonal anti s tag
Figure 1. Intestinal progenitor cells contain P-bodies (A–D) Adult posterior midguts (PMGs) stained for EBs <t>(V5,</t> white), progenitor cells (HRP, green), all cells (DAPI, blue), and either EDC3, GE-1, Me31B, or TRAL (red). (A0)–(D0) are enlargements with EBs labeled (asterisks). (E) PMG stained for TRAL (red), Me31B (green), and DAPI (blue). (F, H, and J) Super-resolution micrographs of progenitor cells stained for EDC3, Me31B, or TRAL pseudocolored using intensity scale in (J); insets are from boxed regions (scale bar, 0.125 mm). (G, I, and K) Pixel-by-pixel intensity profiles along the green dotted line shown in insets. Full genotypes are listed in Data S1F. See also Figure S1.
Resource Source Identifier Antibodies Mouse Monoclonal Anti S Tag, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Intestinal progenitor cells contain P-bodies (A–D) Adult posterior midguts (PMGs) stained for EBs (V5, white), progenitor cells (HRP, green), all cells (DAPI, blue), and either EDC3, GE-1, Me31B, or TRAL (red). (A0)–(D0) are enlargements with EBs labeled (asterisks). (E) PMG stained for TRAL (red), Me31B (green), and DAPI (blue). (F, H, and J) Super-resolution micrographs of progenitor cells stained for EDC3, Me31B, or TRAL pseudocolored using intensity scale in (J); insets are from boxed regions (scale bar, 0.125 mm). (G, I, and K) Pixel-by-pixel intensity profiles along the green dotted line shown in insets. Full genotypes are listed in Data S1F. See also Figure S1.

Journal: Current biology : CB

Article Title: Coordinated repression of pro-differentiation genes via P-bodies and transcription maintains Drosophila intestinal stem cell identity.

doi: 10.1016/j.cub.2021.11.032

Figure Lengend Snippet: Figure 1. Intestinal progenitor cells contain P-bodies (A–D) Adult posterior midguts (PMGs) stained for EBs (V5, white), progenitor cells (HRP, green), all cells (DAPI, blue), and either EDC3, GE-1, Me31B, or TRAL (red). (A0)–(D0) are enlargements with EBs labeled (asterisks). (E) PMG stained for TRAL (red), Me31B (green), and DAPI (blue). (F, H, and J) Super-resolution micrographs of progenitor cells stained for EDC3, Me31B, or TRAL pseudocolored using intensity scale in (J); insets are from boxed regions (scale bar, 0.125 mm). (G, I, and K) Pixel-by-pixel intensity profiles along the green dotted line shown in insets. Full genotypes are listed in Data S1F. See also Figure S1.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-V5 Bio-Rad Cat# MCA1360GA; RRID:AB_567249 Mouse anti-FLAG Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse anti-FMRP DSHB Cat# 5A11; RRID:AB_528252 Mouse anti-Prospero DSHB Cat# MR1A; RRID:AB_528440 Mouse anti-Delta DSHB Cat# C594.9B; RRID:AB_528194 Mouse anti-Me31B Barbee et al.32 N/A Mouse anti-Tubulin DSHB Cat# 12G10; RRID:AB_1157911 Rabbit anti-EDC3 This study N/A Rabbit anti-GFP Thermo Fisher Scientific Cat# A11122; RRID:AB_221569 Rabbit anti-HA Cell Signaling Technology Cat# 3724S; RRID:AB_1549585 Rabbit anti-Me31B Nakamura et al.30 N/A Rabbit anti-GE1 Fan et al.31 N/A Rabbit anti-Pdm1 A gift from Xiaohang Yang N/A Rabbit anti-PATR-1 Pradhan et al.43 N/A Rat anti-TRAL This study N/A Rat anti-EDC3 This study N/A Chemicals, peptides, and recombinant proteins Paraformaldehyde solution Electron Microscopy Sciences Cat# 15714 Bleomycin Calbiochem Cat# 203408 TRIzol LS reagent Ambion Cat# 10296028 Elastase Sigma-Aldrich Cat# E0258 10X PBS pH 7.4, RNase-free Invitrogen Cat# AM9624 Heptane Sigma-Aldrich Cat# 246654 Triton X-100 Sigma-Aldrich Cat# 11332481001 Vectashield Vector Laboratories Cat# H-1000 ProLong Diamond Antifade Invitrogen Cat# P36965 Krebs-Ringer media Alfa Aesar Cat# J67591 Rapamycin LC Laboratories Cat# r-5000 ECL-detection reagent 1 and 2 Thermo Fisher Scientific Cat# 1859701 SUPERasIn RNase Inhibitor Invitrogen Cat# AM2696 cOmplete Protease Inhibitor Roche Cat# 1183617001 Turbo DNase Thermo Fisher Scientific Cat# AM2239 Superscript III Thermo Fisher Scientific Cat# 56575 PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 Critical commercial assays RNAscope Multiplex Fluorescent Reagent Kit v2 ACD Cat# 323100 Ovation SoLo RNA-seq system Tecan Cat# 0502 including 0407 ApopTag Fluorescein In situ Apoptosis Detection Kit Sigma-Aldrich Cat# S7110; RRID:AB_2661855 Deposited data Patr-1 RNA-seq data: GSE183996 This paper GEO: GSE183996 esg RNA-seq data: ArrayExpress E-MTAB-2915 Korzelius et al.51 N/A vtd RNA-seq data: Provided by H. Jasper Khaminets et al.50 N/A (Continued on next page) e1 Current Biology 32, 386–397.e1–e6, January 24, 2022

Techniques: Staining, Labeling